Additional Selective and Differential Cell Culture Media
Cell Applications Inc ENDO CELL BASAL MEDIUM 500ML
ENDO CELL BASAL MEDIUM 500ML Basal Medium without Growth Supplement
Promocell GmbH PromoCell Endothelial Cell Basal Medium MV 2 (prf) 500 ml, phenol red-free
Endothelial Cell Growth Medium MV2 is a low-serum (5% V/V) medium developed for the in vitro cultivation of endothelial cells from microvascular vessels, the coronary artery, and the aorta.The medium lacks the Endothelial Cell Growth Supplement (ECGS, bovine hypothalamic extract) but contains basic Fibroblast Growth Factor, Insulin-like Growth Factor and Vascular Endothelial Growth Factor (VEGF). Because of its effects on cell metabolism, VEGF may interfere with certain experimental setups. In these cases, we recommend the use of Endothelial Cell Growth Medium MV.| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.05 ml / ml || Epidermal Growth Factor (recombinant human) | 5 ng / ml || Basic Fibroblast Growth Factor (recombinant human) | 10 ng / ml || Insulin-like Growth Factor (Long R3 IGF) | 20 ng / ml || Vascular Endothelial Growth Factor 165 (recombinant human) | 0.5 ng / ml || Ascorbic Acid | 1 μg / ml || Hydrocortisone | 0.2 μg / ml |
Promocell GmbH PromoCell M1-Macrophage Generation Medium XF 250 ml, contains: C-28055B, C-39855, C-39894
The PromoCell Macrophage Generation Media have been developed for the efficient generation of monocyte-derived macrophages (MDM) from freshly isolated peripheral blood monocytes or directly from PBMC as a starting material. The M1-Macrophage Generation Medium XF contains GM-CSF and allows for the generation of M1-polarized macrophages. PromoCells M1-Macrophage Generation Medium XF is a ready-to-use medium including cytokines for the directed differentiation of M1-like polarized MDM.The Macrophage Media XF are serum-free and xeno-free formulations for use with freshly isolated cells. Due to the utilization of exclusively synthetic, recombinant or plant-sourced materials, human serum albumin, purified from human plasma, is the only non-recombinant protein contained in this medium.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for murine macrophages.
NEOGEN KF Streptococcus Agar, 5 kg
KF Streptococcus Agar is used with triphenyltetrazolium chloride for the selective isolation and enumeration of fecal streptococci in a laboratory setting. KF Streptococcus Agar is not intended for use in the diagnosis of disease or other conditions in humans.KF Streptococcus Agar was developed by Kenner, Clark, and Kabler for the detection of fecal streptococci in surface waters. KF Streptococcus Agar is currently used in detecting fecal streptococci in water, foods, and other materials through the membrane filtration technique or pour plate method. Fecal streptococci are normal inhabitants in the intestines, and the presence of fecal streptococci can be used as an indication of fecal contamination. The presence of fecal streptococci is of value in determining pollution sources because certain fecal streptococci are host-specific.For more information visit https://www.neogen.com/solutions/Microbiology/kf-streptococcus-agar
Bioworld Murashige African Violet/Gloxinia Multiplication Medium, 50 L
Murashige African Violet/Gloxinia Multiplication MediumContains Macro and Micronutrients as described by Murashige & Skoog (1962) and Vitamins as described by Linsmaier and Skoog (1965) modified with addition of Sodium Phosphate Monobasic, IAA, and KinetinSucrose, Cytokines, and Auxins should be added as needed Provided as 1 unit to make 10L, 25L, or 50L of medium, or as 10 units to make 1L of mediumPlant tissue culture testedWorking Concentration: 4.66 g/LpH: 3.5–4.5Soluble in water
Bioworld Westvaco WV3 Medium, 50 L
Westvaco WV3 MediumCauses a significant improvement in shoot micropropagation Contains Macronutrients, Micronutrients, and Viatmins as described by Coke (1996)Sucrose, Cytokines, and Auxins should be added as needed Provided as 1 unit to make 10L or 50L of mediumPlant tissue culture testedWorking Concentration: 4.29 g/LSoluble in water
Bioworld DKW Basal Medium, w/ 10 g/L Sucrose, 50 L
DKW Basal MediumContains Macronutrients, Micronutrients, and Sucrose as described by Driver & Kuniyuki (1984) and McGranaha et al. (1987)Cytokines, Vitamins, and Auxins should be added as needed Provided as 1 unit to make 10L, 25L, or 50L of medium, or as 10 units to make 1L of mediumPlant tissue culture testedWorking Concentration: 15.22 g/LpH: 3.5–4.5Soluble in water
NEOGEN Perfringens Agar Base (TSC), 10 kg
Perfringens Agar Base (TSC) is used with selective and differential supplements for the recovery of Clostridium perfringens, and is not intended for use in the diagnosis of disease or other conditions in humans. Formulated according to ISO 14189:2013, Perfringens Agar Base (TSC) is a nutritious base medium used in the preparation of SFP Agar and TSC Agar. Depending upon the formula, supplements are added to increase the selectivity of the medium. Shahidi-Ferguson Perfringens (SFP) Agar is based on the formula by Shahidi and Ferguson and incorporates the supplements, Polymyxin B Sulfate and Kanamycin. Tryptose Sulphite Cycloserine (TSC) Agar is developed with the same base as SFP Agar, with the addition of Cycloserine. If desired, Egg Yolk Emulsion can be added to either formula.For more information visit https://www.neogen.com/solutions/Microbiology/perfringens-agar-base
NEOGEN Rappaport Vassiliadis R10 Broth, 500 g
Rappaport Vassiliadis R10 Broth is used for the selective enrichment of Salmonella spp. from foods in a laboratory setting. Rappaport Vassiliadis R10 Broth is not intended for use in the diagnosis of disease or other conditions in humans. Rappaport et al formulated an enrichment medium for Salmonella spp. that was modified by Vassiliadis et al. The Rappaport formulation, designated R 25/37°C, recommended incubation at 37°C. The Vassiliadis modification, designated R 10/43°C, had a reduced level of Malachite Green and recommended incubation at 43°C. Peterz later showed that incubation at 41.5 ± 0.5°C for 24 hours improved recovery of Salmonella spp. Rappaport-Vassiliadis R10 Broth is a selective enrichment medium that is used following pre-enrichment of the specimen.For more information visit https://www.neogen.com/solutions/Microbiology/rappaport-vassiliadis-r10-broth
NEOGEN Dichloran Glycerol (DG-18) Agar Base, 10 kg
Dichloran Glycerol (DG-18) Agar Base is used for the selective isolation and enumeration of yeasts and molds from foods in a laboratory setting. Dichloran Glycerol (DG-18) Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. Dichloran Glycerol (DG-18) Agar Base is used for the enumeration of viable osmophilic yeasts and xerophilic molds in food or animal feed products with a water activity of less than or equal to 0.95. This includes such foods as dry fruits, jams, cakes, dried meat, salted fish, grains, cereals, flours, nuts, spices, condiments and some animal feeds. This medium is not suitable for the examination of dehydrated products with a water activity of greater than 0.95, Dichloran Rose Bengal Chloramphenicol Agar (NCM0082 or NCM0029) should be used.For more information visit https://www.neogen.com/solutions/Microbiology/dichloran-glycerol-agar-base
NEOGEN Lactobacilli MRS Broth, 5 kg
Lactobacilli MRS Broth is used for the cultivation of lactobacilli and is not intended for use in the diagnosis of disease or other conditions in humans.Lactobacilli MRS broth was originally developed in 1960 by de Man, Rogosa & Sharpe, the medium can be used for confirmatory tests on organisms isolated on MRS Agar. The medium can also be used for enumeration by the Miles and Misra technique.Nutrition is provided by a mixture of carefully selected peptones, glucose, beef and yeast extracts while Tween 80, magnesium and manganese sulfates act as growth stimulants. Selectivity against streptococci & molds is provided by ammonium citrate and sodium acetate.For more information visit https://www.neogen.com/solutions/Microbiology/lactobacilli-mrs-broth
NEOGEN Lactobacilli MRS Agar, 10 kg
Lactobacilli MRS Agar is used for the cultivation of lactobacilli and is not intended for use in the diagnosis of disease or other conditions in humans.MRS Agar is a medium for the cultivation and enumeration of Lactobacillus spp. Originally developed in 1960 by de Man, Rogosa & Sharpe, the medium is suitable for most lactic acid bacteria and is intended as a substitute for Tomato Juice Agar. When acidified to pH 5.4 MRS Agar can be used to enumerate Lactobacillus bulgaricus in yogurts.Nutrition is provided by a mixture of carefully selected peptones, glucose, beef & yeast extracts while Tween 80, magnesium and manganese sulfates act as growth stimulants. Selectivity against streptococci & molds is provided by ammonium citrate and sodium acetate. Used at low pH, ammonium citrate allows growth of lactobacilli while inhibiting a number of other microorganism groups.For more information visit https://www.neogen.com/solutions/Microbiology/lactobacilli-mrs-agar
NEOGEN Tryptic Soy Agar with Lecithin and Tween 80, 500 g
Tryptic Soy Agar with Lecithin and Tween 80 is used for the isolation of microorganisms from surfaces sanitized with quaternary ammonium compounds and is not intended for use in the diagnosis of disease or other conditions in humans. In 1955, Leavitt et al. discovered Tryptic Soy Agar supported excellent growth of aerobic and anaerobic microorganisms. Tryptic Soy Agar is a nutritious base and a variety of supplements are added to enhance the medium, including Lecithin and Tween 80. The Lecithin and Tween 80 inactivate some preservatives that may inhibit bacterial growth, reducing “preservative carryover”. Tryptic Soy Agar with Lecithin and Tween 80 is recommended for determining the sanitation efficiency of containers, equipment, and work area (environmental monitoring).For more information visit https://www.neogen.com/solutions/Microbiology/tryptic-soy-agar-with-lecithin-and-tween-80
NEOGEN Plate Count Agar (Standard Methods), 10 kg
Plate Count Agar (Standard Methods Agar) is used for the enumeration of bacteria in water, wastewater, food, and dairy products in a laboratory setting. Plate Count Agar is not intended for use in the diagnosis of disease or other conditions in humans. This formula conforms to ISO 4833-1&2:2013, ISO 17410:2001, American Public Health Association (APHA), Association of Official Analytical Chemists (AOAC) and FDA Bacteriological Analytical Manual. Plate Count Agar (Standard Methods Agar) is free from selective supplements and relatively rich in nutrients, making it ideal for the enumeration of viable organisms, either following a pour plate method, or a surface plate method which can be coupled with a spiral plater. Plate Count Agar (Standard Methods Agar) is also referred to as Tryptone Glucose Yeast Agar. This formula is specified in standard method procedures. For more information visit https://www.neogen.com/solutions/Microbiology/plate-count-agar-standard-methods